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KMID : 0381920090390030253
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Korean Journal of Microscopy
2009 Volume.39 No. 3 p.253 ~ p.260
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Rice Cell Origin Recombinant Human Granulocyte Macrophage Colony-Stimulating Factor (rrhGM-CSF) Could Improve the Wound Healing in Diabetic Hamster
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Han Kyu-Boem
Shin Kil-Sang
Kim Wan-Jong
Han Man-Deuk
Jeong Ji-Joo
Heo Si-Hyun
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Abstract
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GM-CSF is a multipotent growth factor, which also plays an important role during the process of wound healing. rhGMCSF was specifically produced from rice cell culture in our laboratory (Hanson Biotech Co., Ltd, Daejeon). The rrhGMCSF contains more oligosaccharide side chains than any other types of GM-CSF. This work was taken to evaluate the influence on wound healing of rrhGM-CSF in male golden hamsters. Full thickness skin defects of 9 mm in diameter were made in the back of hamsters, and 100 μL ointment containing rrhGM-CSF 50 μg/mL was applied. Control groups were given ointment without rrhGM-CSF. The wound sizes were relatively reduced and skin was well regenerated in the experimental group compared with the control group. Structurally, reepithelialization and architecture of the skin following injury were well accomplished in the experimental group. And also, positive reaction of PCNA of the skin following injury was more prominent in rrhGM-CSF containing ointment treatment group. Since this type of GM-CSF has highly glycosylated side chains, the effectiveness might be retain longer and stable, regarding acceleration of wound healing in the animal model. The present study has important implications for further development of the therapeutic manipulation of wound healing using rrhGM-CSF.
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KEYWORD
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Hamster, rrhGM-CSF, Wound healing
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