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KMID : 0425119990370040285
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Parasites, Hosts and Diseases
1999 Volume.37 No. 4 p.285 ~ p.287
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Changes of splenocyte IFN-γ mRNA synthesis in rats infected with Paragonimus westermani
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Sung-Weon CHO/Jun Kyong CHO
Hye Soo KWON/KyoungHwan JOO/Joon Sang LEE/Sung-Weon CHO
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Abstract
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Changes in the expression level of splenocyte IFN-γ mRNA of Sprague-Dawley (SD) rats infected with Paragonimus westermani were analyzed by competitive reverse transcription-polymerase chain reaction (RT-PCR) followed by southern blot. The template RNA was extracted from the splenocytes of rats infected with 20 metacercariae of P. westermani. The products of competitive RT-PCR were subjected to southern blot and enhanced chemiluminescence (ECL), and analyzed with a densitometer. In comparison with that of uninfected control rat splenocytes (value of 1), the levels of mRNA expression of IFN-γ had changed to 0.747 at 1 week post infection (PI), 0.00175 at 2 week PI, 0.0217 at 3 week PI, 0.194 at 4 week PI and then to 0.537 at 5 week PI. The level at 7 week PI had returned to 1.25, comparable with that of uninfected rats. These results show that, when infected with P. westermani, the levels of IFN-γ mRNA of SD rat splenocytes were remarkably reduced by more than 500 times at 2 week PI and restored to normal level at 7 week PI.
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KEYWORD
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Paragonimus westermani, IFN-γ mRNA, competitive RT-PCR, rat,
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