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KMID : 0425120020400010059
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Parasites, Hosts and Diseases
2002 Volume.40 No. 1 p.59 ~ p.64
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Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs
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Dirk C De Graaf/Dirk C De Graaf
Hans De Coninck/Franz Petry/Ilka B Eeckhout/Johan E Peeters
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Abstract
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A Cryptosporidium parvum sporozoite and oocyst λgt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicity of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cryptosporidium immune calves and not by sera from parasite naive animals.
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KEYWORD
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Cryptosporidium, antigen, gene cloning, zinc-finger, bovine,
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