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KMID : 0425120070450020087
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Parasites, Hosts and Diseases
2007 Volume.45 No. 2 p.87 ~ p.94
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Natural occurrence of Mycobacterium as an endosymbiont of Acanthamoeba isolated from a contact lens storage case
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Yu Hak-Sun
Seol Sung-Yong
Jeong Hae-Jin
Chung Dong-Il
Kong Hyun-Hee
Hong Yeon-Chul
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Abstract
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In this study, we describe Korean isolates of Trichomonas vaginalis infected with double-stranded (ds) RNA virus (TVV). One T. vaginalis isolate infected with TVV IH-2 evidenced weak pathogenicity in the mouse assay coupled with the persistent presence of a dsRNA, thereby indicating a hypovirulence effect of dsRNA in T. vaginalis. Cloning and sequence analysis results revealed that the genomic dsRNA of TVV IH-2 was 4,647 bp in length and evidenced a sequence identity of 80% with the previously-described TVV 1-1 and 1-5, but only a 42% identity with TVV 2-1 and 3 isolates. It harbored 2 overlapping open reading frames of the putative capsid protein and dsRNA-dependent RNA polymerase (RdRp). As previously observed in the TVV isolates 1-1 and 1-5, a conserved ribosomal slippage heptamer (CCUUUUU) and its surrounding sequence context within the consensus 14-nt overlap implied the gene expression of a capsid protein-RdRp fusion protein, occurring as the result of a potential ribosomal frameshift event. The phylogenetic analysis of RdRp showed that the Korean TVV IH-2 isolate formed a compact group with TVV 1-1 and 1-5 isolates, which was divergent from TVV 2-1, 3 and other viral isolates classified as members of the Giardiavirus genus.
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KEYWORD
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Acanthamoeba, Mycobacterium, 18S rRNA, endosymbiont
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