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KMID : 0425120070450040255
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Parasites, Hosts and Diseases
2007 Volume.45 No. 4 p.255 ~ p.266
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Protective effect of lectin from Synadenium carinatum on Leishmania amazonensis infection in BALB/c mice
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Sandra R. AFONSO-CARDOSO
Flavio H. RODRIGUES
Marcio A.B. GOMES
Adriano G. SILVA
Ademir ROCHA
Aparecida H.B. GUIMARAES
Ignes CANDELORO
Silvio FAVORETO Jr.
Marcelo S. FERREIRA
Maria A. de SOUZA
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Abstract
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The protective effect of the Synadenium carinatum latex lectin (ScLL), and the possibility of using it as an adjuvant in murine model of vaccination against American cutaneous leishmaniasis, were evaluated. BALB/c mice were immunized with the lectin ScLL (10, 50, 100 ￥ig/animal) separately or in association with the soluble Leishmania amazonensis antigen (SLA). After a challenge infection with 106 promastigotes, the injury progression was monitored weekly by measuring the footpad swelling for 10 weeks. ScLL appeared to be capable of conferring partial protection to the animals, being most evident when ScLL was used in concentrations of 50 and 100 ￥ig/animal. Also the parasite load in the interior of macrophages showed significant reduction (61.7%) when compared to the control group. With regard to the cellular response, ScLL 50 and 100 ￥ig/animal stimulated the delayed-type hypersensitivity (DTH) reaction significantly (P < 0.05) higher than SLA or SLA plus ScLL 10 weeks after the challenge infection. The detection of high levels of IgG2a and the expression of mRNA cytokines, such as IFN-￥a, IL-12, and TNF-￥a (Th1 profiles), corroborated the protective role of this lectin against cutaneous leishmaniasis. This is the first report of the ScLL effect on leishmaniasis and shows a promising role for ScLL to be explored in other experimental models for treatment of leishmaniasis.
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KEYWORD
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Leishmania amazonensis, cutaneous leishmaniasis, lectin, protective effect, Synadenium carinatum
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