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KMID : 0425120070450040283
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Parasites, Hosts and Diseases
2007 Volume.45 No. 4 p.283 ~ p.285
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Differentially expressed genes of Acanthamoeba castellanii during encystation
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Moon Eun-Kyung
Chung Dong-Il
Kong Hyun-Hee
Hong Yeon-Chul
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Abstract
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To examine the expressed gene profile during encystation of Acanthamoeba castellanii Castellani, we used differentially expressed gene (DGE) screening by RT-PCR with 20 sets of random primers. From this analysis, we found that approximately 16 genes showed upregulation during encystation. We chose 6 genes, which had relatively higher expression levels, for further investigation. Based on homology search in database, DEG2 showed 55% of similarity with xylose isomerase, DEG9 showed 37% of similarity with Na P-type ATPase, and DEG14 showed 77% of similarity with subtilisin-like serine proteinase. DEG3 and DEG26 were identified as hypothetical proteins and DEG25 exhibited no significant similarity to any known protein. Encystation of Acanthamoeba has been suggested to be a process to resist adverse environmental or nutritional conditions. Further characterization studies of these genes may provide us with more information on the encystation mechanism of Acanthamoeba.
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KEYWORD
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Acanthamoeba, differentially expressed gene, encystation
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