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KMID : 0425120070450040295
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Parasites, Hosts and Diseases
2007 Volume.45 No. 4 p.295 ~ p.299
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Loss of infectivity of Neospora caninum oocysts maintained for a prolonged time
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Rosangela Soares UZEDA
Kattyanne De Souza COSTA
Sara Lima SANTOS
Alexandre Moraes PINHEIRO
Maria Angela Ornelas De ALMEIDA
Luis Fernando Pita GONDIM
Milton M. McALLISTER
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Abstract
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The purpose of this study was to investigate whether sporulated Neospora caninum oocysts, which had been stored for 46 mo in a 2% sulfuric acid solution at 4°C, remain morphologically viable and infective to gerbils (Meriones unguiculatus). Six gerbils were orally inoculated with doses of 400 or 1,200 oocysts. Two mo after inoculation, the animals did not show any clinical signs, had no histological lesions, and were seronegative for N. caninum at 1: 50 in an immunofluorescent antibody test. PCR using the brain from each gerbil did not reveal N. caninum specific DNA. We conclude that oocysts preserved for 46 mo are not infective, despite being morphologically intact.
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KEYWORD
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Neospora caninum, oocysts, Mongolian gerbils, Meriones unguiculatus, infectivity
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