|
KMID : 0425120080460040243
|
|
Parasites, Hosts and Diseases
2008 Volume.46 No. 4 p.243 ~ p.246
|
|
|
Reactivity of German Cockroach Allergen, Bla g 2, Peptide Fragments to IgE Antibodies in Patients’ Sera
|
|
Lee Hae-Seok
Hong Chein-Soo
Jeong Kyoung-Yong
Shin Kwang-Hyun
Yi Myung-Hee
Gantulaga Darambazar
Yong Tai-Soon
|
|
|
Abstract
|
|
|
|
Bla g 2 is a cockroach allergen of great importance. This study was conducted to identify IgE-binding epitope(s) of Bla g 2 using the recombinant protein technique. Approximately 50% of tested sera showed IgE reactivity to Pichia-expressed Bla g 2 (PrBla g 2) and E. coli-expressed Bla g 2 (ErBla g 2). Only 5.3% of serum samples showed stronger reactivity to PrBla g 2 than ErBla g 2, indicating that serum was reactive to conformational or carbohydrate epitopes. The full-length and 5 peptide fragments of Bla g 2 were produced in E. coli. All fragments showed IgE-binding activity to the cockroach-allergy patients’ sera. Specifically, peptide fragments of amino acid residue 1-75 and 146-225 appeared to be important for IgE-binding. The information about the IgE-binding epitope of Bla g 2 can aid in the diagnosis and treatment for cockroach allergies.
|
|
|
KEYWORD
|
|
|
Blattella germanica, cockroach, Bla g 2, allergen, epitope
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
    
|