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KMID : 0425120100480040319
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Parasites, Hosts and Diseases
2010 Volume.48 No. 4 p.319 ~ p.324
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Molecular Cloning of Plasmodium vivax Calcium-Dependent Protein Kinase 4
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Choi Kyung-Mi
Kim Jung-Yeon
Moon Sung-Ung
Lee Hyeong-Woo
Sattabongkot Jetsumon
Na Byoung-Kuk
Kim Dae-Won
Suh Eun-Jung
Kim Yeon-Joo
Cho Shin-Hyeong
Lee Ho-Sa
Rhie Ho-Gun
Kim Tong-Soo
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Abstract
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A family of calcium-dependent protein kinases (CDPKs) is a unique enzyme which plays crucial roles in intracellular calcium signaling in plants, algae, and protozoa. CDPKs of malaria parasites are known to be key regulators for stage-specific cellular responses to calcium, a widespread secondary messenger that controls the progression of the parasite. In our study, we identified a gene encoding Plasmodium vivax CDPK4 (PvCDPK4) and characterized its molecular property and cellular localization. PvCDPK4 was a typical CDPK which had well-conserved N-terminal kinase domain and C-terminal calmodulin-like structure with 4 EF hand motifs for calcium-binding. The recombinant protein of EF hand domain of PvCDPK4 was expressed in E. coli and a 34 kDa product was obtained. Immunofluorescence assay by confocal laser microscopy revealed that the protein was expressed at the mature schizont of P. vivax. The expression of PvCDPK4-EF in schizont suggests that it may participate in the proliferation or egress process in the life cycle of this parasite.
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KEYWORD
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Plasmodium vivax, calcium-dependent protein kinase, schizont, EF-hand motif
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