KMID : 0425120110490010079
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Parasites, Hosts and Diseases 2011 Volume.49 No. 1 p.79 ~ p.83
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Identification of Antigenic Proteins in Trichomonas vaginalis
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Lee Hye-Yeon
Hyung Su-Jin Lee Jong-Woong Kim Ju-Ri Shin Myeong-Heon Ryu Jae-Sook Park Soon-Jung
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Abstract
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Trichomoniasis is a sexually transmitted disease due to infection with Trichomonas vaginalis, and it can cause serious consequences for women¡¯s health. To study the virulence factors of this pathogen, T. vaginalis surface proteins were investigated using polyclonal antibodies specific to the membrane fractions of T. vaginalis. The T. vaginalis expression library was constructed by cloning the cDNA derived from mRNA of T. vaginalis into a phage ¥ë Uni-ZAP XR vector, and then used for immunoscreening with the anti-membrane proteins of T. vaginalis antibodies. The immunoreactive proteins identified included adhesion protein AP65-1, ¥á-actinin, kinesin-associated protein, teneurin, and 2 independent hypothetical proteins. Immunofluorescence assays showed that AP65-1, one of the identified immunogenic clones, is prevalent in the whole body of T. vaginalis. This study led us to identify T. vaginalis proteins which may stimulate immune responses by human cells.
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KEYWORD
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Trichomonas vaginalis, antigenic protein, membrane protein, immunoscreening
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