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KMID : 0425120110490020177
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Parasites, Hosts and Diseases
2011 Volume.49 No. 2 p.177 ~ p.180
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Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells
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Jang Yun-Soo
Song Kyoung-Ju
Kim Ju-Young
Lee Young-Ah
Kim Kyeong-Ah
Lee Sang-Kyou
Shin Myeong-Heon
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Abstract
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Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and μ-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and μ-calpain may be involved in colon epithelial cell death induced by E. histolytica.
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KEYWORD
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Entamoeba histolytica, host cell death, calpain, HT-29 colon epithelial cells
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