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KMID : 0425120130510020197
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Parasites, Hosts and Diseases
2013 Volume.51 No. 2 p.197 ~ p.201
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Molecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1
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Li Wen Hui
Jia Wan Zhong
Qu Zi Gang
Xie Zhi Zhou
Luo Jian Xun
Yin Hong
Sun Xiao Lin
Blaga Radu
Fu Bao Quan
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Abstract
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A total of 16 Taenia multiceps isolates collected from naturally infected sheep or goats in Gansu Province, China were characterized by sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The complete cox1 gene was amplified for individual T. multiceps isolates by PCR, ligated to pMD18T vector, and sequenced. Sequence analysis indicated that out of 16 T. multiceps isolates 10 unique cox1 gene sequences of 1,623 bp were obtained with sequence variation of 0.12-0.68%. The results showed that the cox1 gene sequences were highly conserved among the examined T. multiceps isolates. However, they were quite different from those of the other Taenia species. Phylogenetic analysis based on complete cox1 gene sequences revealed that T. multiceps isolates were composed of 3 genotypes and distinguished from the other Taenia species.
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KEYWORD
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Taenia multiceps, cytochrome c oxidase subunit 1 (cox1), genotype, phylogeny, China
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