|
KMID : 0425120130510050511
|
|
Parasites, Hosts and Diseases
2013 Volume.51 No. 5 p.511 ~ p.517
|
|
|
Phylogenetic Analysis of Ruminant Theileria spp. from China Based on 28S Ribosomal RNA Gene
|
|
Gou Huitian
Guan Guiquan
Ma Miling
Liu Aihong
Liu Zhijie
Xu Zongke
Ren Qiaoyun
Li Youquan
Yang Jifei
Chen Ze
Yin Hong
Luo Jianxun
|
|
|
Abstract
|
|
|
|
Species identification using DNA sequences is the basis for DNA taxonomy. In this study, we sequenced the ribosomal large-subunit RNA gene sequences (3,037-3,061 bp) in length of 13 Chinese Theileria stocks that were infective to cattle and sheep. The complete 28S rRNA gene is relatively difficult to amplify and its conserved region is not important for phylogenetic study. Therefore, we selected the D2-D3 region from the complete 28S rRNA sequences for phylogenetic analysis. Our analyses of 28S rRNA gene sequences showed that the 28S rRNA was useful as a phylogenetic marker for analyzing the relationships among Theileria spp. in ruminants. In addition, the D2-D3 region was a short segment that could be used instead of the whole 28S rRNA sequence during the phylogenetic analysis of Theileria, and it may be an ideal DNA barcode.
|
|
|
KEYWORD
|
|
|
Theileria sp., 28S rRNA, phylogeny, cattle, sheep, China
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|