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KMID : 0425120140520010021
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Parasites, Hosts and Diseases
2014 Volume.52 No. 1 p.21 ~ p.26
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Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening
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Fu Yan
Lan Jingchao
Wu Xuhang
Yang Deying
Zhang Zhihe
Nie Huaming
Hou Rong
Zhang Runhui
Zheng Wanpeng
Xie Yue
Yan Ning
Yang Zhi
Wang Chengdong
Luo Li
Liu Li
Gu Xiaobin
Wang Shuxian
Peng Xuerong
Yang Guangyou
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Abstract
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Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.
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KEYWORD
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Dirofilaria immitis, heartworm, dirofilariasis, translationally controlled tumor protein, transcriptome
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