KMID : 0425120140520030311
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Parasites, Hosts and Diseases 2014 Volume.52 No. 3 p.311 ~ p.315
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In Vitro Trypanocidal Activity of Macela (Achyrocline satureioides) Extracts against Trypanosoma evansi
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Baldissera Matheus D.
Oliveira Camila B. Zimmermann Carine E. P Boligon Aline A. Athayde Margareth Linde Bolzan Leandro P. Vaucher Rodrigo de A. Santurio Janio M. Sagrillo Michele R. Silva Aleksandro Schafer da Monteiro Silvia G.
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Abstract
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The aim of this study was to verify the trypanocidal effectiveness of aqueous, methanolic, and ethanolic extracts of Achyrocline satureioides against Trypanosoma evansi in vitro. A. satureioides extracts, known as macela, were used on trypomastigotes at different concentrations (1, 5, 10, 50, 100, 500, and 1,000 ¥ìg/ml) and exposure times (0, 1, 3, 6, and 9 hr). A dose-dependent effect was observed when the 3 extracts were tested. The concentrations of 1, 5, and 10 ¥ìg/ml were not able to kill trypomastigotes until 3 hr after exposure, and the highest concentrations (500 and 1,000 ¥ìg/ml) were able to kill all trypomastigotes after 1 hr. When the time of exposure was increased up to 9 hr, the concentrations at 50 and 100 ¥ìg/ml were 100% effective to 3 extracts. The chemical analysis of the extracts revealed the presence of flavonoids, a trypanocidal compound already described. Based on the results, we can conclude that the A. satureioides extracts exhibit trypanocidal effects.
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KEYWORD
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Achyrocline satureioides, Trypanosoma evansi, in vitro effect, flavonoid
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