|
KMID : 0425120140520060613
|
|
Parasites, Hosts and Diseases
2014 Volume.52 No. 6 p.613 ~ p.619
|
|
|
Flavonoids Modulate the Proliferation of Neospora caninum in Glial Cell Primary Cultures
|
|
Matos Rosan Barbosa de
Braga-de-Souza Suzana
Pitanga Bruno Pena Seara
Silva Victor Diogenes Amaral da
Jesus Erica Etelvina Viana de
Pinheiro Alexandre Morales
Costa Maria de Fatima Dias
El-Bacha Ramon dos Santos
Ribeiro Catia Suse de Oliveira
Costa Silvia Lima
|
|
|
Abstract
|
|
|
|
Neospora caninum (Apicomplexa; Sarcocystidae) is a protozoan that causes abortion in cattle, horses, sheep, and dogs as well as neurological and dermatological diseases in dogs. In the central nervous system of dogs infected with N. caninum, cysts were detected that exhibited gliosis and meningitis. Flavonoids are polyphenolic compounds that exhibit antibacterial, antiparasitic, antifungal, and antiviral properties. In this study, we investigated the effects of flavonoids in a well-established in vitro model of N. caninum infection in glial cell cultures. Glial cells were treated individually with 10 different flavonoids, and a subset of cultures was also infected with the NC-1 strain of N. caninum. All of the flavonoids tested induced an increase in the metabolism of glial cells and many of them increased nitrite levels in cultures infected with NC-1 compared to controls and uninfected cultures. Among the flavonoids tested, 3’,4’-dihydroxyflavone, 3’,4’,5,7-tetrahydroxyflavone (luteolin), and 3,3’,4’,5,6-pentahydroxyflavone (quercetin), also inhibited parasitophorous vacuole formation. Taken together, our findings show that flavonoids modulate glial cell responses, increase NO secretion, and interfere with N. caninum infection and proliferation.
|
|
|
KEYWORD
|
|
|
Neospora caninum, glia, flavonoid, nitric oxide (NO)
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|
|