|
KMID : 0425120150530020237
|
|
Parasites, Hosts and Diseases
2015 Volume.53 No. 2 p.237 ~ p.242
|
|
|
Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb
|
|
Oh Chang-Seok
Seo Min
Hong Ji-Won
Chai Jong-Yil
Oh Seung-Whan
Park Jun-Bum
Shin Dong-Hoon
|
|
|
Abstract
|
|
|
|
Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples.
|
|
|
KEYWORD
|
|
|
Ascaris, ancient DNA, cytochrome b, 18S rRNA, Korean mummy
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|