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KMID : 0425120150530060789
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Parasites, Hosts and Diseases
2015 Volume.53 No. 6 p.789 ~ p.793
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Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)
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Tiantian Zhang
Xuejiao Cui
Hui Wang
Hua Zeng
Yuanyuan Cao
Jingze Liu
Yonghong Hu
Jincheng Zhang
Meng Wu
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Abstract
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In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.
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KEYWORD
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Dermacentor silvarum, tick, antigenic protein, LC-MS/MS
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