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KMID : 0425120160540030375
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Parasites, Hosts and Diseases
2016 Volume.54 No. 3 p.375 ~ p.380
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Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection
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Mu-Xin Chen
Jia-Xu Chen
Shao-Hong Chen
Da-Na Huang
Lin Ai
Ren-Li Zhang
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Abstract
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Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.
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KEYWORD
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Angiostrongylus cantonensis, lateral flow immunoassay, monoclonal antibody, antigen detection
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