|
KMID : 0425120160540060725
|
|
Parasites, Hosts and Diseases
2016 Volume.54 No. 6 p.725 ~ p.732
|
|
|
Characterization of Caveola-Vesicle Complexes (CVCs) Protein, PHIST/CVC-8195 in Plasmodium vivax
|
|
Wang Bo
Lu Feng
Han Jin-Hee
Lee Seong-Kyun
Cheng Yang
Nyunt Myat Htut
Ha Kwon-Soo
Hong Seok-Ho
Park Won-Sun
Han Eun-Taek
|
|
|
Abstract
|
|
|
|
Plasmodium vivax produces numerous caveola-vesicle complex (CVC) structures beneath the membrane of infected erythrocytes. Recently, a member helical interspersed subtelomeric (PHIST) superfamily protein, PcyPHIST/CVC-8195, was identified as CVCs-associated protein in Plasmodium cynomolgi and essential for survival of this parasite. Very little information has been documented to date about PHIST/CVC-8195 protein in P. vivax. In this study, the recombinant PvPHIST/CVC-8195 N and C termini were expressed, and immunoreactivity was assessed using confirmed vivax malaria patients sera by protein microarray. The subcellular localization of PvPHIST/CVC-8195 N and C termini in blood stage parasites was also determined. The antigenicity of recombinant PvPHIST/CVC-8195 N and C terminal proteins were analyzed by using serum samples from the Republic of Korea. The results showed that immunoreactivities to these proteins had 61% and 43% sensitivity and 96.9% and 93.8% specificity, respectively. The N terminal of PvPHIST/CVC-8195 which contains transmembrane domain and export motif (PEXEL; RxLxE/Q/D) produced CVCs location throughout the erythrocytic-stage parasites. However, no fluorescence was detected with antibodies against C terminal fragment of PvPHIST/CVC-8195. These results suggest that the PvPHIST/CVC-8195 is localized on the CVCs and may be immunogenic in natural infection of P. vivax.
|
|
|
KEYWORD
|
|
|
Plasmodium vivax, PvPHIST/CVC-8195, caveola-vesicle complex, immunoreactivity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|