|
KMID : 0425120170550020149
|
|
Parasites, Hosts and Diseases
2017 Volume.55 No. 2 p.149 ~ p.158
|
|
|
Diversity of vir Genes in Plasmodium vivax from Endemic Regions in the Republic of Korea: an Initial Evaluation
|
|
Son Ui-Han
Dinzouna Boutamba Sylvatrie Danne
Lee Sang-Hyun
Yun Hae-Soo
Kim Jung-Yeon
Joo So-Young
Jeong Soo-Kwan
Rhee Man-Hee
Hong Yeon-Chul
Chung Dong-Il
Kwak Dong-Mi
Goo Youn-Kyoung
|
|
|
Abstract
|
|
|
|
Variant surface antigens (VSAs) encoded by pir families are considered to be the key proteins used by many Plasmodium spp. to escape the host immune system by antigenic variation. This attribute of VSAs is a critical issue in the development of a novel vaccine. In this regard, a population genetic study of vir genes from Plasmodium vivax was performed in the Republic of Korea (ROK). Eighty-five venous blood samples and 4 of the vir genes, namely vir 27, vir 21, vir 12, and vir 4, were selected for study. The number of segregating sites (S), number of haplotypes (H), haplotype diversity (Hd), DNA diversity (π and Θw), and Tajima’s D test value were conducted. Phylogenetic trees of each gene were constructed. The vir 21 (S=143, H=22, Hd=0.827) was the most genetically diverse gene, and the vir 4 (S=6, H=4, Hd=0.556) was the opposite one. Tajima’s D values for vir 27 (1.08530, P>0.1), vir 12 (2.89007, P<0.01), and vir 21 (0.40782, P>0.1) were positive, and that of vir 4 (?1.32162, P>0.1) was negative. All phylogenetic trees showed 2 clades with no particular branching according to the geographical differences and cluster. This study is the first survey on the vir genes in ROK, providing information on the genetic level. The sample sequences from vir 4 showed a clear difference to the Sal-1 reference gene sequence, whereas they were very similar to those from Indian isolates.
|
|
|
KEYWORD
|
|
|
Plasmodium vivax, vir gene, genetic diversity, the Republic of Korea
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|
|