KMID : 0425120170550040439
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Parasites, Hosts and Diseases 2017 Volume.55 No. 4 p.439 ~ p.444
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Syphacia obvelata: A New Hope to Induction of Intestinal Immunological Tolerance in C57BL/6 Mice
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Taghipour Niloofar
Mosaffa Nariman Rostami Nejad Mohammad Homayoni Mohamad Mohsen Mortaz Esmaeil Aghdaei Hamid Asadzadeh Zali Mohammad Reza
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Abstract
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The ability of nematodes to manipulate the immune system of their host towards a Th2 and T regulatory responses has been proposed to suppress the inflammatory response. Clinical trials have proposed a useful effect of helminth infections on improvement of inflammatory disorders. In this study, we investigated the immunomodulatory effect of Syphacia obvelata infection to induce intestinal tolerance in C57BL/6 mice. Mice were infected through the cagemates with self-infected BALB/c mice. Four weeks post-infection, expression levels of IFN-¥ã, TNF-¥á, IL-17, and IL-10 were assessed in the supernatant of mesenteric lymph node (MLN) culture. Foxp3+Treg were measured in MLN cells by flow cytometry. In the S. obvelata-infected group, the percentage of Tregs (5.2¡¾0.4) was significantly higher than the control (3.6¡¾0.5) (P<0.05). The levels of IL-10 (55.3¡¾2.2 vs 35.2¡¾3.2), IL-17 (52.9¡¾3.8 vs 41¡¾1.8), IFN-¥ã (44.8¡¾4.8 vs 22.3¡¾2.3) and TNF-¥á (71.1¡¾5.8 vs 60.1¡¾3.3) were significantly increased in infected mice compared to the control group (P<0.05). The above results showed the potential effects of S. obvelata to induce intestinal tolerance. Therefore, it seems that S. obvelata may increase the immunological suppressive function in the intestinal tract.
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KEYWORD
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Syphacia obvelata, T regulatory cell, Foxp3, C57BL/6, inflammatory disorder, intestinal tolerance
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