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KMID : 0425120170550060613
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Parasites, Hosts and Diseases
2017 Volume.55 No. 6 p.613 ~ p.622
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IL-12 and IL-23 Production in Toxoplasma gondii- or LPS Treated Jurkat T Cells via PI3K and MAPK Signaling Pathways
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Ismail Hassan Ahmed Hassan Ahmed
Kang Byung-Hun
Kim Jae-Su
Lee Jae-Hyung
Choi In-Wook
Cha Guang-Ho
Yuk Jae-Min
Lee Young-Ha
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Abstract
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IL-12 and IL-23 are closely related in structure, and have been shown to play crucial roles in regulation of immune responses. However, little is known about the regulation of these cytokines in T cells. Here, we investigated the roles of PI3K and MAPK pathways in IL-12 and IL-23 production in human Jurkat T cells in response to Toxoplasma gondii and LPS. IL-12 and IL-23 production was significantly increased in T cells after stimulation with T. gondii or LPS. T. gondii and LPS increased the phosphorylation of AKT, ERK1/2, p38 MAPK, and JNK1/2 in T cells from 10 min post-stimulation, and peaked at 30-60 min. Inhibition of the PI3K pathway reduced IL-12 and IL-23 production in T. gondii-infected cells, but increased in LPS-stimulated cells. IL-12 and IL-23 production was significantly reduced by ERK1/2 and p38 MAPK inhibitors in T. gondii- and LPS-stimulated cells, but not in cells treated with a JNK1/2 inhibitor. Collectively, IL-12 and IL-23 production was positively regulated by PI3K and JNK1/2 in T. gondii-infected Jurkat cells, but negatively regulated in LPS-stimulated cells. And ERK1/2 and p38 MAPK positively regulated IL-12 and IL-23 production in Jurkat T cells. These data indicate that T. gondii and LPS induced IL-12 and IL-23 production in Jurkat T cells through the regulation of the PI3K and MAPK pathways; however, the mechanism underlying the stimulation of IL-12 and IL-23 production by T. gondii in Jurkat T cells is different from that of LPS.
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KEYWORD
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Toxoplasma gondii, LPS, IL-12, IL-23, PI3K/AKT, MAPK pathway
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