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KMID : 0425120170550060679
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Parasites, Hosts and Diseases
2017 Volume.55 No. 6 p.679 ~ p.684
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Practical Algorisms for PCR-RFLP-Based Genotyping of Echinococcus granulosus Sensu Lato
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Kim Hye-Jin
Yong Tae-Soon
Shin Myeong-Heon
Lee Kyu-Jae
Park Gab-Man
Suvonkulov Uktamjon
Kovalenko Dmitriy
Yu Hak-Sun
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Abstract
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Echinococcus granulosus sensu lato (s.l.) is a causative agent of cystic echinococcosis or cystic hydatid disease in humans and domestic and wild animals. The disease is a serious health problem in countries associated with poverty and poor hygiene practices, particularly in livestock raising. We introduced a practical algorism for genotyping the parasite, which may be useful to many developing countries. To evaluate the efficiency of the algorism, we genotyped 3 unknown strains isolated from human patients. We found that unknowns 1 and 3 were included in G1, G2, and G3 genotypes group and unknown 2 was included in G4 genotype (Echinococcus equinus) according to the algorisms. We confirmed these results by sequencing the 3 unknown isolates cox1 and nad1 PCR products. In conclusion, these new algorisms are very fast genotype identification tools that are suitable for evaluating E. granulosus s.l. isolated from livestock or livestock holders, particularly in developing countries.
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KEYWORD
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Echinococcus granulosus, practical algorism, genotyping, cox1, nad1
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