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KMID : 0425120180560020113
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Parasites, Hosts and Diseases
2018 Volume.56 No. 2 p.113 ~ p.119
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Detection of Cryptosporidium spp. in Diarrheic Immunocompetent Patients in Beni-Suef, Egypt: Insight into Epidemiology and Diagnosis
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Gawad Samah S. Abdel
Ismail Mousa A. M.
Imam Naglaa F. A.
Eassa Ahmed H. A.
abu-Sarea Enas Yahia
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Abstract
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Cryptosporidium species is an important cause of gastrointestinal infections globally. This study aimed to shed light on its role in diarrheic immunocompetent patients in Beni-Suef, Egypt and to compare three diagnostic methods. Two hundred diarrheic patients, 37±16.8 year old, were enrolled. Stool samples were examined by light microscopy, using modified Ziehl-Neelsen stain (MZN) for Cryptosporidium spp. oocysts. Coproantigens were detected by sandwich ELISA. DNA molecular diagnosis was done by nested PCR. PCR yielded the highest detection rates (21.0%), compared to ELISA (12.5%) and MZN staining method (9.5%). The higher infection rates were in 20?40 year-old group, followed by 40?60 year-old. Association between epidemiologic factors was statistically not significant; positivity and gender, clinical manifestations, residence, source or water, or contact with animals. Cryptosporidiosis is an important enteric parasitic infection in Beni-Suef and PCR remains the gold standard for diagnosis.
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KEYWORD
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Cryptosporidium, Beni-Suef, PCR, ELISA, modified Ziehl-Neelsen stain
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