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KMID : 0425120180560020121
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Parasites, Hosts and Diseases
2018 Volume.56 No. 2 p.121 ~ p.127
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Detection and Identification of Sarcocystis cruzi (Protozoa: Apicomplexa) by Molecular and Ultrastructural Studies in Naturally Infected Korean Cattle (Bos taurus coreanae) from Daejeon, Korea
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Choi Tong-Il
Hong Eui-Ju
Ryu Si-Yun
Sim Cheol-Ho
Chae Joon-Seok
Kim Hyeon-Cheol
Park Jin-Ho
Choi Kyoung-Seong
Yu Do-Hyeon
Yoo Jae-Gyu
Park Bae-Keun
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Abstract
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To survey the prevalence of Sarcocystis infections, 210 heart samples were collected from Korean native cattle (Bos taurus coreanae) at an abattoir in Daejeon Metropolitan City, Republic of Korea. Sarcocysts were detected form 31 specimens (14.8%) and identified as Sarcocystis cruzi via transmission electron microscopy. The wall of S. cruzi has flattened protrusions that did not contain fibrils or microfilaments. The protrusions arose irregularly from the base, contained a fine granular substance, lacked internal microfilaments, and measured approximately 0.21?1.25 μm in length and 0.05?0.07 μm in width. Sequence analysis revealed 99.5% homology to S. cruzi. This is the first report on the prevalence of S. cruzi in native cattle from the Republic of Korea.
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KEYWORD
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Sarcocystis cruzi, Bos taurus coreanae, ultrastructure, 18S rRNA
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