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KMID : 0425120180560030275
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Parasites, Hosts and Diseases
2018 Volume.56 No. 3 p.275 ~ p.280
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Genetic and Morphologic Identification of Spirometra ranarum in Myanmar
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Jeon Hyeong-Kyu
Park Han-Sol
Lee Dong-Min
Choe Seong-Jun
Kang Ye-Seul
Bia Mohammed Mebarek
Lee Sang-Hwa
Sohn Woon-Mok
Hong Sung-Jong
Chai Jong-Yil
Eom Kee-Seon S.
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Abstract
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In the present study, we identified a Spirometra species of Myanmar origin (plerocercoid) by molecular analysis using mitochondrial cox1 and nad1 genes, as well as by morphological observations of an adult tapeworm. Spargana specimens were collected from a paddy-field in Taik Kyi Township Tarkwa Village, Yangon, Myanmar in December 2017. A total of 5 spargana were obtained from 20 frogs Hoplobatrachus rugulosus; syn: Rana rugulosa (Wiegmann, 1834) or R. tigrina (Steindachner, 1867). The plerocercoids were used for experimental infection of a dog. After 4 weeks of infection, an adult tapeworm was recovered from the intestine of the dog. Morphologically, the distinct features of Spirometra sp. (Myanmar origin) relative to S. erinaceieuropaei and S. decipiens include a uterine morphology comprising posterior uterine coils that larger than the terminal uterine ball and coiling of the uteri diagonally (swirling) rather than spirally. The cox1 sequences (1,566 bp) of the Myanmar-origin Spirometra species showed 97.9% similarity to a reference sequence of S. decipiens (GenBank no. KJ599679) and 90.5% similarity to a reference sequence of S. erinaceieuropaei (GenBank no. KJ599680). Phylogenetic tree topologies were identical and presented high confidence level of values for the 3 major branches of the 3 Spirometra species in cox1 and nad1 genes. These results indicated that Myanmar-origin Spirometra species coincided with those of S. ranarum and may be considered as a valid species.
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KEYWORD
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Spirometra ranarum, spargana, molecular detection, cox1, nad1, Myanmar
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