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KMID : 0425120180560050437
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Parasites, Hosts and Diseases
2018 Volume.56 No. 5 p.437 ~ p.446
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Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets
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Yoo Won-Gi
Kim Sun-Min
Won Eun-Jeong
Lee Ji-Yun
Dai Fuhong
Woo Ho-Choon
Nam Ho-Woo
Kim Tae-Im
Han Jeong-Hee
Kwak Dong-Mi
Cho Yun-Sang
Kang Seung-Won
Kim Tong-Soo
Zhu Xing-Quan
Wang Chunren
Youn Hee-Jeong
Hong Sung-Jong
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Abstract
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To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066?0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.
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KEYWORD
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Toxoplasma gondii, ELISA, pork, tissue fluid, Korean, imported
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