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KMID : 0425120190570010027
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Parasites, Hosts and Diseases
2019 Volume.57 No. 1 p.27 ~ p.31
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Comparison of Two PCR Assays for Trichomonas vaginalis
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Noh Chang-Suk
Kim Sang-Su
Park Sung-Yul
Moon Hong-Sang
Hong Yeon-Chul
Ryu Jae-Sook
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Abstract
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PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.
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KEYWORD
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Trichomonas vaginalis, PCR, DNA isolation
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