|
KMID : 0425120190570020083
|
|
Parasites, Hosts and Diseases
2019 Volume.57 No. 2 p.83 ~ p.92
|
|
|
Dipenyleneiodonium Induces Growth Inhibition of Toxoplasma gondii through ROS Induction in ARPE-19 Cells
|
|
Sun Pu-Reum
Gao Fei Fei
Choi Hei-Gwon
Zhou Wei
Yuk Jae-Min
Kwon Jae-Yul
Lee Young-Ha
Cha Guang-Ho
|
|
|
Abstract
|
|
|
|
Based on the reactive oxygen species (ROS) regulatory properties of diphenyleneiodonium (DPI), we investigated the effects of DPI on host-infected T. gondii proliferation and determined specific concentration that inhibit the intracellular parasite growth but without severe toxic effect on human retinal pigment epithelial (ARPE-19) cells. As a result, it is observed that host superoxide, mitochondria superoxide and H2O2 levels can be increased by DPI, significantly, followed by suppression of T. gondii infection and proliferation. The involvement of ROS in anti-parasitic effect of DPI was confirmed by finding that DPI effect on T. gondii can be reversed by ROS scavengers, N-acetyl-L-cysteine and ascorbic acid. These results suggest that, in ARPE-19 cell, DPI can enhance host ROS generation to prevent T. gondii growth. Our study showed DPI is capable of suppressing T. gondii growth in host cells while minimizing the un-favorite side-effect to host cell. These results imply that DPI as a promising candidate material for novel drug development that can ameliorate toxoplasmosis based on ROS regulation.
|
|
|
KEYWORD
|
|
|
T. gondii, reactive oxygen species, diphenyleneiodonium, N-acetyl-L-cysteine, ARPE-19 cell
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
    
|