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KMID : 0425120190570020201
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Parasites, Hosts and Diseases
2019 Volume.57 No. 2 p.201 ~ p.206
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Production of Inflammatory Cytokines and Nitric Oxide by Human Mast Cells Incubated with Toxoplasma gondii Lysate
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Park Eun-Ah
Han Ik-Hwan
Kim Jung-Hyun
Park Soon-Jung
Ryu Jae-Sook
Ahn Myoung-Hee
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Abstract
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The roles of mast cells in allergic diseases and helminth infections are well known. However, the roles of mast cells in T. gondii infection is poorly understood. This study was focused on the production of pro-inflammatory cytokines (TNF-α, IL-4), chemokines (CXCL8, MCP-1) and nitric oxide (NO) by mast cells in response to soluble lysate of T. gondii tachyzoites. Production of CXCL8 (IL-8), MCP-1, TNF-α and IL-4 were measured by RT-PCR and ELISA. Western blot were used for detection of CXCR-1 and CXCR2. Our results showed that T. gondii lysates triggered mast cells to release CXCL8, MCP-1, TNF-α, IL-4 and to produce NO. This suggests that mast cells play an important role in inflammatory responses to T. gondii.
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KEYWORD
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Toxoplasma gondii, mast cell, cytokine, nitric oxide
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