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KMID : 0425120200580010087
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Parasites, Hosts and Diseases
2020 Volume.58 No. 1 p.87 ~ p.91
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Morphological and Molecular Confirmation of Parvatrema duboisi Metacercariae in the Manila Clam Ruditapes philippinarum from Gochang-gun, Korea
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Chang Tae-Hee
Jung Bong-Kwang
Shin Hye-Joo
Hong Soo-Ji
Lee Jeong-Gyu
Kim Deok-Gyu
Patarwut Laddawan
Sohn Woon-Mok
Chai Jong-Yil
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Abstract
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Gymnophallid metacercariae found in the Manila clam Ruditapes philippinarum (‘Banjirak’ in Korean) from Gochang-gun, Jeollabuk-do, Korea were morphologically and molecularly confirmed to be Parvatrema duboisi (Dollfus, 1923) Bartoli, 1974. The metacercariae were morphologically characterized by having a large oral sucker, small ventral sucker, genital pore some distance anterior to the ventral sucker, no ventral pit, and 1 compact or slightly lobed vitellarium, which were all compatible with P. duboisi. Some of the metacercariae were experimentally fed to mice, and adult flukes were recovered at day 7 post-infection. The morphology of the adult flukes was basically the same as that of the metacercariae except for the presence of uterine eggs; the uterus was filled with up to 40 eggs. The nucleotide sequences (1,193 bp) from ITS regions (ITS1, 5.8S rDNA, and ITS2) of the metacercariae showed 99.7% identity with P. duboisi and 75.7% identity with Gymnophalloides seoi deposited in GenBank. These results confirmed the presence of P. duboisi metacercariae in the Manila clam R. philippinarum in an estuary region of Gochang-gun, Korea.
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KEYWORD
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Parvatrema duboisi, gymnophallid, Manila clam, Gochang-gun, Korea
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