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KMID : 0425120210590060615
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Parasites, Hosts and Diseases
2021 Volume.59 No. 6 p.615 ~ p.623
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Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid
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Lu Yan
Sun Jia-Hui
Lu Li-Li
Chen Jia-Xu
Song Peng
Ai Lin
Cai Yu-Chun
Li Lan-Hua
Chen Shao-Hong
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Abstract
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Human sparganosis is a food-borne parasitic disease caused by the plerocercoids of Spirometra species. Clinical diagnosis of sparganosis is crucial for effective treatment, thus it is important to identify sensitive and specific antigens of plerocercoids. The aim of the current study was to identify and characterize the immunogenic proteins of Spirometra erinaceieuropaei plerocercoids that were recognized by patient sera. Crude soluble extract of the plerocercoids were separated using 2-dimensional gel electrophoresis coupled with immunoblot and mass spectrometry analysis. Based on immunoblotting patterns and mass spectrometry results, 8 antigenic proteins were identified from the plerocercoid. Among the proteins, cysteine protease protein might be developed as an antigen for diagnosis of sparganosis.
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KEYWORD
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Spirometra erinaceieuropaei, plerocercoid, immunoproteomic, diagnosis, antigen
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