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KMID : 0425120220600030163
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Parasites, Hosts and Diseases
2022 Volume.60 No. 3 p.163 ~ p.172
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Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia
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Park Eun-Ah
Kim Ju-Ri
Shin Mee-Young
Park Soon-Jung
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Abstract
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Kinesin-13 (Kin-13), a depolymerizer of microtubule (MT), has been known to affect the length of Giardia. Giardia Kin-13 (GlKin-13) was localized to axoneme, flagellar tips, and centrosomes, where phosphorylated forms of Giardia polo-like kinase (GlPLK) were distributed. We observed the interaction between GlKin-13 and GlPLK via co-immunoprecipitation using transgenic Giardia cells expressing Myc-tagged GlKin-13, hemagglutinin-tagged GlPLK, and in vitro-synthesized GlKin-13 and GlPLK proteins. In vitro-synthesized GlPLK was demonstrated to auto-phosphorylate and phosphorylate GlKin-13 upon incubation with [γ-32P]ATP. Morpholino-mediated depletion of both GlKin-13 and GlPLK caused an extension of flagella and a decreased volume of median bodies in Giardia trophozoites. Our results suggest that GlPLK plays a pertinent role in formation of flagella and median bodies by modulating MT depolymerizing activity of GlKin-13.
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KEYWORD
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Giardia lamblia, GlKin-13, GlPLK, flagella, median body
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