KMID : 0425120230610030317
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Parasites, Hosts and Diseases 2023 Volume.61 No. 3 p.317 ~ p.324
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Standard- and large-sized eggs of Trichuris trichiura in the feces of schoolchildren in the Yangon Region, Myanmar: Morphological and molecular analyses
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Ryoo Seung-Wan
Jung Bong-Kwang Hong Soo-Ji Shin Hye-Joo Song Hye-Mi Kim Hyun-Seung Ryu Jin-Youp Sohn Woon-Mok Hong Sung-Jong Thi Thi Htoon Chai Jong-Yil
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Abstract
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Standard- and large-sized eggs of Trichuris trichiura were found in the feces of schoolchildren in Yangon, Myanmar during epidemiological surveys and mass deworming with albendazole in 2017-2019. The standard-sized eggs were identified as those of T. trichiura, but it was necessary to exclude the possibility of the large-sized eggs belonging to Trichuris vulpis, a dog whipworm. We conducted morphological and molecular studies to determine the species of the 2 types of Trichuris eggs. Individual eggs of both sizes were isolated from Kato-Katz fecal smears (n=20) and mechanically destroyed using a 23G injection needle. Nuclear DNA was extracted, and the 18S rRNA region was sequenced in 15 standard-sized eggs and 15 large-sized eggs. The average size of standard-sized eggs (T. trichiura) was 55.2¡¿26.1 ¥ìm (range: 51.7-57.6¡¿21.3-28.0 ¥ìm; n=97), whereas the size of large-sized eggs was 69.3¡¿32.0 ¥ìm (range: 65.1-76.4¡¿30.1-34.5 ¥ìm; n=20), slightly smaller than the known size of T. vulpis. Regarding standard-sized eggs, the 18S rRNA nucleotide sequences exhibited 100% homology with T. trichiura deposited in GenBank and 88.6-90.5% homology with T. vulpis. Regarding large-sized eggs, the nucleotide sequences showed 99.8-100% homology with T. trichiura in GenBank and 89.6- 90.7% homology with T. vulpis. Both standard- and large-sized eggs of Trichuris spp. found in Myanmar schoolchildren during 2017-2019 were morphologically and molecularly confirmed to belong to T. trichiura. The conversion of eggs from smaller to large sizes might be due to anthelmintic treatments with albendazole.
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KEYWORD
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Trichuris trichiura, Trichuris vulpis, nematode, egg size, 18S rRNA, Myanmar
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