KMID : 0525720200250010024
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Journal of Chitin and Chitosan 2020 Volume.25 No. 1 p.24 ~ p.28
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Recovery of Chitin-Glucan Complex in By-products Obtained from Industrial Yeast Fermentation
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Seo Dong-Jun
Song Yong-Su Jo Gyeong-Hyun Moon Chae-Yeong Jung Woo-Jin
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Abstract
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Chitin-glucan complex (CGC) copolymer is a cell wall component in fungi and yeasts, used in medicine and cosmetics applications. This study investigated the production of CGC from Aureobasidium pullulans KSY-0516, which is used in the industrial production of ¥â-1,3/1,6-glucan. The amino acid composition of the A. pullulans fermentation by-products was determined using an amino acid analyzer. The total amount of amino acid was 6.67 g/100 g of by-product. Additionally, the recovery of the ¥â-glucan complex from the A. pullulans fermentation by-products was 8.44 g/100 g of by-product using ethanol precipitation. The CGC yield was determined from the by-products after yeast fermentation. The recovery of CGC was 14.83% for the yeast cell wall obtained by alkaline extraction. The CGC and ¥â-glucan were characterized using Fourier transform infrared analysis, and compared with crab shell chitin and laminarin. This study aimed to examine the potential, as a useful substrate for biotechnology, by producing CGC from yeast cultivation by-products.
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KEYWORD
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Chitin-glucan complex, ¥â-Glucan, Chitin, By-products
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