KMID : 0545119960060060397
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Journal of Microbiology and Biotechnology 1996 Volume.6 No. 6 p.397 ~ p.401
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Production of Cycloinulooligosaccharide Fructanotransferase (CFTase) from Bacillus sp. CFC1
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KIM, HWA-YOUNG
PARK, JEONG-BOK/KWON, YOUNG-MAN/CHOI, YONG-JIN
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Abstract
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A bacterial strain CFC1, which produced an extracellular cycloinulooligosaccharide fructanotransferase (CFTase), was isolated from soil. The isolated strain was identified as a strain of BAcillus sp. The synthesis of CFTase by the bacterium was found to be induced by inulin which was added to the culture medium as a carbon source. The highest activity of CFTase was observed at pH 7.5 and 37¡É in the medium containing 4% inulin and 0.5% peptone as a carbon source and a nitrogen source, respectively. Under the optimal conditions, the enzyme activity in the culture supernatant reached the highest level of 85 munits/ml after 96 h cultivation.
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