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KMID : 0545120010110010079
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 1 p.79 ~ p.84
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Plasmid-Mediated Aniline Assimilation by Pseudomonas sp. B10
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El-Deeb Bahig A.
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Abstract
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An aniline-utilizing microorganism identified as a species of Pseudomonas was isolated from soil contaminated highly with aniline and urea-herbicide. This strain was able to utilize aniline as the sole source of carbon and energy, and was shown to harbor a single large plasmid mediating the aniline assimilation. Subsequent plasmid-curing of this bacterium resulted in the abolishment of the aniline utilizing phenotype and the loss of catechol-C2, 3O-oxygenase. The reestablishment of the plasmid, denoted pB10, in cured Pseudomonas sp. via filter surface mating, resulted in restoration of the aniline assimilation abilities and enzyme activity.
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