KMID : 0545120040140020395
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Journal of Microbiology and Biotechnology 2004 Volume.14 No. 2 p.395 ~ p.400
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An Immobilized Culture of Choristoneura fumiferana Cells for High Production of CfMNPV
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SON, JEONG HWA
BUCHHOLIZ, RAINER/KIM, SUNG-KOO
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Abstract
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Choristnneuru fumiferanu (Cf-2C 1 ) insect cells were cultured and immobilized by using cellulosesulfate (NaCS) and polydiallyldimethylammoniumchloride (PDADMAC). A concentration of¢¥ CNNPV (Chorisroneuru fumiferLznu multiple- nucleopolyhedrovirus) and a Cf-2C I cell density in the microspheres have been achieved at the densities of 1.57x 10^(10) PIBs/ml and 7.5¡¿10^(7)cells/ml, respectively. Additionally, MTT-test was used to measure the viable cell density in the microspheres, and the confidence of MTT-test was investigated before and after baculovirus infection in the immobilized cell culture.
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