KMID : 0545120150250101761
|
|
Journal of Microbiology and Biotechnology 2015 Volume.25 No. 10 p.1761 ~ p.1767
|
|
Development of a Recombinant Protein Vaccine Based on Cell-Free Protein Synthesis for Sevenband Grouper Epinephelus septemfasciatus Against Viral Nervous Necrosis
|
|
Kim Jong-Oh
Kim Jae-Ok Kim Wi-Sik Oh Myung-Joo
|
|
Abstract
|
|
|
Sevenband grouper, Epinephelus septemfasciatus, is becoming an important aquaculture species in Korea. However, viral nervous necrosis disease is a large problem causing mass mortality in sevenband grouper aquaculture. Recombinant protein vaccines are one of the best methods to reduce these economic losses. However, the cell-based expression method mainly produces inclusion bodies and requires additional procedures. In this study, we expressed a recombinant viral coat protein of sevenband grouper nervous necrosis virus (NNV) using a cell-free protein synthesis system. The purified recombinant NNV coat protein (rNNV-CP) was injected into sevenband grouper at different doses followed by a NNV challenge. Nonimmunized fish in the first trial (20 ¥ìg/fish) began to die 5 days post-challenge and reached 70% cumulative mortality. In contrast, immunized fish also starting dying 5 days postchallenge but lower cumulative mortality (10%) was observed. Cumulative morality in the second trial with different doses (20, 4, and 0.8 ¥ìg/fish) was 10%, 40%, and 50%, respectively. These results suggest that rNNV-CP can effectively immunize sevenband grouper depending on the dose administered. This study provides a new approach to develop a recombinant vaccine against NNV infection for sevenband grouper.
|
|
KEYWORD
|
|
viral nervous necrosis (VNN), nervous necrosis virus (NNV), cell free protein synthesis (CFPS), recombinant protein, sevenband grouper
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|