KMID : 0545120170270111932
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Journal of Microbiology and Biotechnology 2017 Volume.27 No. 11 p.1932 ~ p.1941
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In Vitro Effect of DFC-2 on Mycolic Acid Biosynthesis in Mycobacterium tuberculosis
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Kim Su-Kyung
Seo Hoon-Hee Mahmud Hafij Al Islam Imtiazul Kim Yong-Sik Lyu Ji-Won Nam Kung-Woo Lee Byung-Eui Lee Kee-In Song Ho-Yeon
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Abstract
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DFC-2, a methyl 5-[2-(dimethylamino)ethoxy]-7,12-dioxo-7,12-dihydrodinaphtho[1,2-b:2¡¯,3¡¯- d]furan-6-carboxylate, is reported to have antitubercular effects against Mycobacterium tuberculosis. At concentrations ranging from 0.19 to 0.39 ¥ìg/ml, DFC-2 inhibited both drugsusceptible and ?resistant strains of M. tuberculosis. Microarray analyses were employed to gain insights into the molecular mechanisms of DFC-2¡¯s action in M. tuberculosis. The most affected functional gene category was ¡°lipid biosynthesis,¡± which is involved in mycolic acid synthesis. The decrease in transcription of genes related to mycolic acid synthesis was confirmed by RT-PCR. Furthermore, we found that DFC-2 triggered a reduction in mycolic acid levels, showing a similar pattern to that of mycolic acid synthesis inhibitor isoniazid. These results may explain how this compound kills mycobacteria efficiently by inhibiting mycolic acid synthesis.
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KEYWORD
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DFC-2, mycolic acid, M. tuberculosis
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