KMID : 0545120170270112070
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Journal of Microbiology and Biotechnology 2017 Volume.27 No. 11 p.2070 ~ p.2073
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A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase
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Lee Jin-Moo
Cho Jin-Beom Ahn Hee-Chul Jung Woong Jeong Yong-Joo
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Abstract
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We have discovered a novel chemical compound, (E)-3-(furan-2-yl)-N-(4-sulfamoylphenyl) acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we obtained IC50 values of 2.09 ¡¾ 0.30 ¥ìM (ATP hydrolysis) and 13.2 ¡¾ 0.9 ¥ìM (DNA unwinding), respectively. Moreover, we found that the compound did not have any significant cytotoxicity when 40 ¥ìM of it was used. Our results showed that the compound might be useful to be developed as an inhibitor against SARS coronavirus.
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KEYWORD
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SARS, helicase, ATP hydrolysis, dsDNA unwinding, cytotoxicity
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