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KMID : 0545120190290081193
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Journal of Microbiology and Biotechnology
2019 Volume.29 No. 8 p.1193 ~ p.1203
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Protective Effect of 3-Bromo-4,5-Dihydroxybenzaldehyde from Polysiphonia morrowii Harvey against Hydrogen Peroxide-Induced Oxidative Stress In Vitro and In Vivo
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Cho Su-Hyeon
Heo Soo-Jin
Yang Hye-Won
Ko Eun-Yi
Jung Myeong-Seon
Cha Seon-Heui
Ahn Gin-Nae
Jeon You-Jin
Kim Kil-Nam
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Abstract
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We investigated the protective effects of 3-bromo-4,5-dihydroxybenzaldehyde (BDB) from Polysiphonia morrowii Harvey against hydrogen peroxide (H2O2)-induced apoptosis in Vero cells. BDB exhibited scavenging activity for DPPH, hydroxyl, and alkyl radicals. BDB also inhibited H2O2-induced lipid peroxidation, cell death, and apoptosis in Vero cells by inhibiting the production of ROS. To evaluate the molecular mechanisms of apoptosis inhibition, the expression of Bax/Bcl-xL and NF-κB was assessed by western blot assay. BDB significantly suppressed the cleavage of caspase-9 and PARP and reduced Bax levels in H2O2-induced Vero cells. Besides, BDB suppressed the phosphorylation of NF-κB and the translocation of p65 in H2O2-induced cells. Furthermore, we evaluated the effect of BDB on ROS production, cell death, and lipid peroxidation in an H2O2-stimulated zebrafish embryo model. Taken together, these results indicated that ROS generation and cell death were significantly inhibited by BDB in zebrafish embryos, thereby proving that BDB exerts excellent antioxidant activity in vitro and in vivo.
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KEYWORD
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Polysiphonia morrow Harvey, 3-bromo-4,5-dihydroxybenzaldehyde, reactive oxygen species, oxidative stress, antioxidant, zebrafish embryos
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