KMID : 0545120230330101361
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Journal of Microbiology and Biotechnology 2023 Volume.33 No. 10 p.1361 ~ p.1369
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Crystal Structures of 6-Phosphogluconate Dehydrogenase from Corynebacterium glutamicum
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Yu Hyeon-Jeong
Hong Ji-Yeon Seok Ji-Hye Seu Young-Bae Kim Il-Kwon Kim Kyung-Jin
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Abstract
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Corynebacterium glutamicum (C. glutamicum) has been considered a very important and meaningful industrial microorganism for the production of amino acids worldwide. To produce amino acids, cells require nicotinamide adenine dinucleotide phosphate (NADPH), which is a biological reducing agent. The pentose phosphate pathway (PPP) can supply NADPH in cells via the 6-phosphogluconate dehydrogenase (6PGD) enzyme, which is an oxidoreductase that converts 6-phosphogluconate (6PG) to ribulose 5-phosphate (Ru5P), to produce NADPH. In this study, we identified the crystal structure of 6PGD_apo and 6PGD_NADP from C. glutamicum ATCC 13032 (Cg6PGD) and reported our biological research based on this structure. We identified the substrate binding site and co-factor binding site of Cg6PGD, which are crucial for understanding this enzyme. Based on the findings of our research, Cg6PGD is expected to be used as a NADPH resource in the food industry and as a drug target in the pharmaceutical industry.
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KEYWORD
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6-Phosphogluconate dehydrogenase, Corynebacterium glutamicum, crystal structure, 6-phosphogluconate, nicotinamide adenine dinucleotide phosphate
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