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KMID : 0578319900010010021
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Molecules and Cells
1990 Volume.1 No. 1 p.21 ~ p.24
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Identification of the Transcription Regulatory Sequences for the Nitrogen Catabolite Repression of Yeast ASP3 Gene
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Jeong Yoo-Jin
Kim Kyu-Won
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Abstract
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Asparaginase II negative Saccharomyces cerevlSlae strain, XE223-1D, was transformed with plasmids pKW1 (8.2-kb insert), pKW2 (4.2-kb insert) and pKW3 (4.0-kb insert) which contain the ASP3 gene and its flanking sequences. Plasmids pKW1 and pKW3 conferred a normal level of asparaginase II biosynthesis and a normal nitrogen catabolite repression (NCR) to their host strain. In contrast, plasmid pKW2 was partially resistant to NCR. Plasmid pKW2 carries a small deletion (350 bp) in the upstream region of the ASP3 gene. Therefore, we suggest that NCR-regulatory sequences of the ASP3 gene are located in the upstream region of the gene.
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KEYWORD
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