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KMID : 0578319900010010093
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Molecules and Cells
1990 Volume.1 No. 1 p.93 ~ p.98
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Effect of Amino Acid Substitutions at a Site of Temperature Sensitive Folding Mutation of P22 Tailspike Protein
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Park Joo-Sang
Koh Hye-Yeong
Yu Myeong-Hee
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Abstract
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Twelve different single amino acid substitutions were made at the site of a temperature sensitive folding mutation, TsfU2 (Asp238→Ser), of the phage P22 tailspike protein. Most of the substitutions except Pro and Arg did not affect folding and maturation of the tailspike protein at 28 ℃. However, at 39 ℃ only the wild type residue, Asp, and the Thr substitution allowed the formation of the tailspike trimers. The results suggest that a stereospecific interaction of the residue 238 is required for the folding and maturation of the tailspike, which is critical for stabilizing the folding intermediate at high temperature.
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