|
KMID : 0578319910010020137
|
|
Molecules and Cells
1991 Volume.1 No. 2 p.137 ~ p.143
|
|
|
Expression of Tomato Proteinase Inhibitor I Confers Tobacco Plants Resistance against Insects
|
|
Lee, Jong Seob
Moon, Youngho/Choi, Yeonhee
|
|
|
Abstract
|
|
|
|
Plants have been considered to defend themselves from attacking pests by synthesizing proteinase inhibitors and storing them in storage organs such as seeds and tubers. Their synthesis is also induced in leaves of tomato and potato when attacked by insects. In order to investigate possible roles of proteinase inhibitors in plants we tried to express a wound-inducible proteinase inhibitor I gene of tomato in tobacco plants constitutively. A chimeric gene between the promoter of the cauliflower mosaic virus (CaMV) 35 S transcript and cDNA of the tomato inhibitor I gene was constructed in a binary vector derived from Ti-plasmid of Agrobacterium tumefaciens. The CaMV 35S-inhibitor I gene was introduced into tobacco cells by the method of Agrobacterium-mediated transformation. Transformed cells were regenerated to whole plants. Immunoassay of leaf extracts from the transgenic tobacco plants with anti-inhibitor I antibody confirmed the expression of the inhibitor I gene in the transgenic plants. Insect bioassay with the transgenic plants indicated that the tobacco plants synthesizing the inhibitor I proteins are mostly protected from insect attacks while control plants are not.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|