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KMID : 0578319910010020151
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Molecules and Cells
1991 Volume.1 No. 2 p.151 ~ p.156
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Molecualr Cloning of the Pectate Lyase gene from Rhizobium fredeii USDA193
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Lim, Sun Tech
Chung, Min Hwa/Park, Yong Woo/Yun, Han Dae
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Abstract
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Rhizobium fredii USDA193 is a microsymbiont of Glycine max (soybeans) which was originally isolated from Peoples Republic of China. It infects and nodulates soybean roots via root hairs by actively penetrating the root hair cell-wall. The penetration of Rhizobiwn into the root hair cell-wall requires the activity of cell-wall hydrolyzing enzymes such as cellulases and pectinases. Evidence is presented that R fredii USDA193 produces pectate lyase enzymes and that their genetic determinants are borne on the resident symbiotic plasmid (pSym) DNA. The pectate lyase genes peB and pelf of Erwinia chrysanthemi EC16 show homology to a 4.0-kb EcoRI fragment of pSym DNA. The peB and pelf homologues in R fredii USDA193 are tandem)y located on the 0.7-kb Sall HindIII and the 1.3-kb HindIII EcoRI fragments, respectively. These two subfragments carrying the homologous RpelB and RpelE (of Rhizobium pel) genes, have been subcloned into the vectors, pUC18 and pUC19. The clones independently express pectate lyase enzyme activity in an Escherichia soli background.
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KEYWORD
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