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KMID : 0578319910010020163
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Molecules and Cells
1991 Volume.1 No. 2 p.163 ~ p.168
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Mechanism of Drug Resistance in Hepatoma tissues
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Kim, Eung Gook
Kwak, Sahng June/Park, Sang Schul/Kim, Soo Tae
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Abstract
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The biochemical basis of the multi-drug resistance is recently described in terms of the increased expression of genes for P-glycoprotein and phase 11 enzymes such as gammaglutamyl transpeptidase (GGTP) and glutathione S-transferase (GST). Northern blot analysis was carried out for GGTP. GST-P, and P-glycoprotein gene expressions in the normal and neoplastic murine liver tissues induced by Solt/Farber procedure, in murine primary hepatocyics of 5-day cultures, and in the human normal liver and hepatoma tissues, to study the molecular mechanism of general intrinsic drug-resistance in hepatoma tissues. Transcriptions of GGTP. GST-P and mdr genes increased in the aged primary cultured hepatocytes, while only those of GGTP and GST-P were elevated in murine neoplastic liver tissues. In contrast, any correlation between the transcriptions of these and P-glycoprotein gene was not observed in human liver tissues. These results suggest that transcriptions of GST-P. GGTP and ,ndr- genes are related with the general cullular adaptive response to the environmental changes, rather than with carcinogenic process itself. Moreover, the primary cultured murine hepatocytes appears to he an in vitro model system for the study on cxpresson of´ these genes.
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