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KMID : 0578319910010020183
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Molecules and Cells
1991 Volume.1 No. 2 p.183 ~ p.186
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Relationship between Inositol Phospholipid Metabolism and Initiation of DNA synthesis during Early S Phase of Cell Cycle
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Lee, Myung Ae
Joe, cheol O./Chung, Jae Hoon/Kang, Ke Won
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Abstract
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Intracellular inositol phospholipid hydrolysis involved in the initiation of DNA synthesis was examined. NIH 3T3 cells metabolically labeled with [´H]inositol were growth-arrested by serum starvation and 3 mM hydroxyurea treatment, and cells were allowed to initiate DNA synthesis in the normal media. The increased breakdown of inositol phospholipid into inositol phosphates and diacylglycerol was observed preceding to the maximal DNA synthesis which was measured by the [´H]thymidine incorporation into DNA of cells during the progression of cell into S phase. Phosphorylation of phosphatidylinositol into phosphatidylinositol phosphates subsequent to the hydrolysis into inositol phosphates began to increase about 2 h before the onset of maximal DNA synthesis. These results suggest that the change of inositol phospholipid metabolism is closely associated with the initiation of DNA synthesis.
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